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PMID:17916790
| Citation |
Li, Y and Schellhorn, HE (2007) Rapid kinetic microassay for catalase activity. J Biomol Tech 18:185-7 |
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| Abstract |
Catalase is a commonly assayed enzyme found in many bacteria and eukaryotes. In this report, we examined the applicability of a kinetic microassay to quantify catalase from two different sources. The assay was found to be linear over a wide range (0.1-1.0 units), but was limited at high values (>1 unit) by oxygen evolution. Nonetheless, the microassay allows simultaneous evaluation of many samples (up to 96) in a short time (<5 min) and is thus well-suited to applications, such as high-throughput screening, where many parallel assays are required. |
| Links | |
| Keywords |
Catalase/genetics; Catalase/metabolism; Escherichia coli/enzymology; Kinetics; Sensitivity and Specificity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0004096: catalase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0004096: catalase activity |
ECO:0000315: |
F |
Fig. 3 Deletions show reduced catalase activity |
complete | ||||
See also
References
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