GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:17894411
| Citation |
Wolf, M, Nunes, F, Henkel, A, Heinick, A and Paul, RJ (2008) The MAP kinase JNK-1 of Caenorhabditis elegans: location, activation, and influences over temperature-dependent insulin-like signaling, stress responses, and fitness. J. Cell. Physiol. 214:721-9 |
|---|---|
| Abstract |
The mitogen-activated protein kinase (MAPK) pathways and insulin-like signaling play pivotal roles in cellular stress response. Using an anti-phospho-SAPK/JNK antibody and a daf-16::GFP-based reporter assay, the present study shows in Caenorhabditis elegans that ambient temperature (1-37 degrees C) specifically influences the activation (phosphorylation) of the MAP kinase JNK-1 as well as the nuclear translocation of DAF-16, the main downstream target of insulin-like signaling. Activated JNK-1 was detected only in neuronal cells, and JNK-1 was found to be controlled by the MAPK JKK-1 under heat stress. Comparative analyses on the wildtype and a jnk-1 deletion mutant revealed a promoting influence of JNK-1 on both nuclear DAF-16 translocations and DAF-16 target gene (superoxide dismutase 3, sod-3) expressions within peripheral, non-neuronal tissue. Consequently, the mutant exhibited a reduced thermal tolerance and reproductive fitness at higher temperatures. These results provide evidence of indirect interactions between neuronal MAPK and peripheral insulin-like signaling in response to environmental stimuli (temperature, H2O2). |
| Links |
PubMed Online version:10.1002/jcp.21269 |
| Keywords |
Animals; Caenorhabditis elegans/cytology; Caenorhabditis elegans/drug effects; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/metabolism; Cell Nucleus/drug effects; Cell Nucleus/enzymology; Enzyme Activation/drug effects; Gene Deletion; Hydrogen Peroxide/pharmacology; Insulin/metabolism; Intestines/cytology; Intestines/drug effects; Intestines/enzymology; Isoenzymes/metabolism; Mitogen-Activated Protein Kinases/metabolism; Protein Transport/drug effects; Reproduction/drug effects; Signal Transduction/drug effects; Superoxide Dismutase/metabolism; Temperature; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
