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PMID:17890223
| Citation |
Nelson, MR, Luo, H, Vari, HK, Cox, BJ, Simmonds, AJ, Krause, HM, Lipshitz, HD and Smibert, CA (2007) A multiprotein complex that mediates translational enhancement in Drosophila. J. Biol. Chem. 282:34031-8 |
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| Abstract |
Modulating the efficiency of translation plays an important role in a wide variety of cellular processes and is often mediated by trans-acting factors that interact with cis-acting sequences within the mRNA. Here we show that a cis-acting element, the Hsp83 degradation element (HDE), within the 3'-untranslated region of the Drosophila Hsp83 mRNA functions as a translational enhancer. We show that this element is bound by a multiprotein complex, and we identify components using a novel affinity-based method called tandem RNA affinity purification tagging. Three proteins (DDP1, Hrp48, and poly(A)-binding protein) are components of the HDE-binding complex and function in translational enhancement. Our data support a model whereby the HDE is composed of several cis-acting subelements that represent binding sites for trans-acting factors, and the combined action of these trans-acting factors underlies the ability of the HDE to stimulate translation. |
| Links |
PubMed Online version:10.1074/jbc.M706363200 |
| Keywords |
3' Untranslated Regions/genetics; 3' Untranslated Regions/metabolism; Animals; Binding Sites/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Drosophila Proteins/biosynthesis; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster; Heat-Shock Proteins/biosynthesis; Heat-Shock Proteins/genetics; Heterogeneous-Nuclear Ribonucleoproteins/genetics; Heterogeneous-Nuclear Ribonucleoproteins/metabolism; Multiprotein Complexes/genetics; Multiprotein Complexes/metabolism; Poly(A)-Binding Proteins/genetics; Poly(A)-Binding Proteins/metabolism; Protein Biosynthesis/physiology; RNA Stability/physiology |
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Significance
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