PMID:17722983

Citation	Bussmann, J, Bakkers, J and Schulte-Merker, S (2007) Early endocardial morphogenesis requires Scl/Tal1. PLoS Genet. 3:e140
Abstract	The primitive heart tube is composed of an outer myocardial and an inner endocardial layer that will give rise to the cardiac valves and septa. Specification and differentiation of these two cell layers are among the earliest events in heart development, but the embryonic origins and genetic regulation of early endocardial development remain largely undefined. We have analyzed early endocardial development in the zebrafish using time-lapse confocal microscopy and show that the endocardium seems to originate from a region in the lateral plate mesoderm that will give rise to hematopoietic cells of the primitive myeloid lineage. Endocardial precursors appear to rapidly migrate to the site of heart tube formation, where they arrive prior to the bilateral myocardial primordia. Analysis of a newly discovered zebrafish Scl/Tal1 mutant showed an additional and previously undescribed role of this transcription factor during the development of the endocardium. In Scl/Tal1 mutant embryos, endocardial precursors are specified, but migration is severely defective and endocardial cells aggregate at the ventricular pole of the heart. We further show that the initial fusion of the bilateral myocardial precursor populations occurs independently of the endocardium and tal1 function. Our results suggest early separation of the two components of the primitive heart tube and imply Scl/Tal1 as an indispensable component of the molecular hierarchy that controls endocardium morphogenesis.
Links	PubMed PMC1950955 Online version:10.1371/journal.pgen.0030140
Keywords	Animals; Animals, Genetically Modified; Base Sequence; Basic Helix-Loop-Helix Transcription Factors/genetics; Basic Helix-Loop-Helix Transcription Factors/physiology; Codon, Nonsense; Embryo, Nonmammalian; Endocardium/embryology; Endocardium/metabolism; Models, Biological; Molecular Sequence Data; Morphogenesis/genetics; Myoblasts, Cardiac/cytology; Myoblasts, Cardiac/metabolism; Phylogeny; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/physiology; Receptors, Vascular Endothelial Growth Factor/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish Proteins/genetics; Zebrafish Proteins/physiology
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