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PMID:17720867
| Citation |
Kaiserli, E and Jenkins, GI (2007) UV-B promotes rapid nuclear translocation of the Arabidopsis UV-B specific signaling component UVR8 and activates its function in the nucleus. Plant Cell 19:2662-73 |
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| Abstract |
Arabidopsis thaliana UV RESISTANCE LOCUS8 (UVR8) is a UV-B-specific signaling component that binds to chromatin and regulates UV protection by orchestrating expression of a range of genes. Here, we studied how UV-B regulates UVR8. We show that UV-B stimulates the nuclear accumulation of both a green fluorescent protein (GFP)-UVR8 fusion and native UVR8. Nuclear accumulation leads to UV-B induction of the HY5 gene, encoding a key transcriptional effector of the UVR8 pathway. Nuclear accumulation of UVR8 is specific to UV-B, occurs at low fluence rates, and is observed within 5 min of UV-B exposure. Attachment of a nuclear export signal (NES) to GFP-UVR8 causes cytosolic localization in the absence of UV-B. However, UV-B promotes rapid nuclear accumulation of NES-GFP-UVR8, indicating a concerted mechanism for nuclear translocation. UVR8 lacking the N-terminal 23 amino acids is impaired in nuclear translocation. Attachment of a nuclear localization signal (NLS) to UVR8 causes constitutive nuclear localization. However, NLS-GFP-UVR8 only confers HY5 gene expression following UV-B illumination, indicating that nuclear localization, although necessary for UVR8 function, is insufficient to cause expression of target genes; UV-B is additionally required to stimulate UVR8 function in the nucleus. These findings provide new insights into the mechanisms through which UV-B regulates gene expression in plants. |
| Links |
PubMed PMC2002606 Online version:10.1105/tpc.107.053330 |
| Keywords |
Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/radiation effects; Arabidopsis Proteins/metabolism; Cell Nucleus/metabolism; Cell Nucleus/radiation effects; Chromosomal Proteins, Non-Histone/metabolism; Green Fluorescent Proteins/metabolism; Kinetics; Light; Models, Biological; Mutation/genetics; Nuclear Export Signals; Plants, Genetically Modified; Promoter Regions, Genetic/genetics; Protein Transport/radiation effects; Recombinant Fusion Proteins/metabolism; Ultraviolet Rays |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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