PMID:17656141

Citation	Sabourin, M, Tuzon, CT and Zakian, VA (2007) Telomerase and Tel1p preferentially associate with short telomeres in S. cerevisiae. Mol. Cell 27:550-61
Abstract	In diverse organisms, telomerase preferentially elongates short telomeres. We generated a single short telomere in otherwise wild-type (WT) S. cerevisiae cells. The binding of the positive regulators Ku and Cdc13p was similar at short and WT-length telomeres. The negative regulators Rif1p and Rif2p were present at the short telomere, although Rif2p levels were reduced. Two telomerase holoenzyme components, Est1p and Est2p, were preferentially enriched at short telomeres in late S/G2 phase, the time of telomerase action. Tel1p, the yeast ATM-like checkpoint kinase, was highly enriched at short telomeres from early S through G2 phase and even into the next cell cycle. Nonetheless, induction of a single short telomere did not elicit a cell-cycle arrest. Tel1p binding was dependent on Xrs2p and required for preferential binding of telomerase to short telomeres. These data suggest that Tel1p targets telomerase to the DNA ends most in need of extension.
Links	PubMed PMC2650483 Online version:10.1016/j.molcel.2007.07.016
Keywords	Chromosomes, Fungal/genetics; Chromosomes, Fungal/metabolism; DNA, Circular/metabolism; Intracellular Signaling Peptides and Proteins/metabolism; Multiprotein Complexes/metabolism; Protein Binding; Protein-Serine-Threonine Kinases/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Telomerase/metabolism; Telomere/genetics; Telomere/metabolism
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