PMID:17634240

Citation	Murray, CL, Jones, CT, Tassello, J and Rice, CM (2007) Alanine scanning of the hepatitis C virus core protein reveals numerous residues essential for production of infectious virus. J. Virol. 81:10220-31
Abstract	Hepatitis C virus (HCV) is an important human pathogen affecting an estimated 3% of the world's population. Recent advances have enabled in vitro propagation of the virus and allow assembly and egress to be investigated for the first time. As a component of the virion, the HCV core protein likely functions primarily in infectious virus production, although little is known about the determinants of this activity. To investigate the roles of core in the viral life cycle, we performed a comprehensive deletion and alanine scanning mutagenesis study of this protein in the context of a genotype 2a reporter virus. We have confirmed that core protein is essential for infectious virion production and have identified numerous residues required for this role. The infectivity of several assembly-defective core mutants could be rescued by compensatory mutations identified in p7 and NS2, suggesting genetic interactions with core and highlighting the importance of these nonstructural proteins in infectious virion morphogenesis.
Links	PubMed PMC2045476 Online version:10.1128/JVI.00793-07
Keywords	Alanine/chemistry; Alanine/genetics; Amino Acid Sequence; Amino Acid Substitution; Cell Line; Genome, Viral/genetics; Hepacivirus/genetics; Hepacivirus/physiology; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Serine/chemistry; Serine/genetics; Viral Core Proteins/genetics; Viral Core Proteins/physiology; Virion/genetics; Virion/physiology; Virus Replication/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HCVJ6:POLG		GO:0016032: viral reproduction	ECO:0000315:		P		Figure 5	complete CACAO 1996
HCVJ6:POLG	involved_in	GO:0016032: viral process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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