PMID:17626846

Citation	Ding, M, Chao, D, Wang, G and Shen, K (2007) Spatial regulation of an E3 ubiquitin ligase directs selective synapse elimination. Science 317:947-51
Abstract	Stereotyped synaptic connectivity can arise both by precise recognition between appropriate partners during synaptogenesis and by selective synapse elimination. The molecular mechanisms that underlie selective synapse removal are largely unknown. We found that stereotyped developmental elimination of synapses in the Caenorhabditis elegans hermaphrodite-specific motor neuron (HSNL) was mediated by an E3 ubiquitin ligase, a Skp1-cullin-F-box (SCF) complex composed of SKR-1 and the F-box protein SEL-10. SYG-1, a synaptic adhesion molecule, bound to SKR-1 and inhibited assembly of the SCF complex, thereby protecting nearby synapses. Thus, subcellular regulation of ubiquitin-mediated protein degradation contributes to precise synaptic connectivity through selective synapse elimination.
Links	PubMed Online version:10.1126/science.1145727
Keywords	Animals; Caenorhabditis elegans/growth & development; Caenorhabditis elegans/metabolism; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Cell Cycle Proteins/metabolism; Immunoglobulins/genetics; Immunoglobulins/metabolism; Models, Neurological; Motor Neurons/physiology; Mutation; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Oviposition; Promoter Regions, Genetic; Proteasome Endopeptidase Complex/metabolism; R-SNARE Proteins/metabolism; RNA Interference; Recombinant Fusion Proteins/metabolism; SKP Cullin F-Box Protein Ligases/metabolism; Synapses/physiology; Two-Hybrid System Techniques
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