PMID:17586488

Citation	Etard, C, Behra, M, Fischer, N, Hutcheson, D, Geisler, R and Strähle, U (2007) The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis. Dev. Biol. 308:133-43
Abstract	Contraction of muscles is mediated by highly organized arrays of myosin motor proteins. We report here the characterization of a mutation of a UCS gene named steif/unc-45b that is required for the formation of ordered myofibrils in both the skeletal and cardiac muscles of zebrafish. We show that Steif/Unc-45b interacts with the chaperone Hsp90a in vitro. The two genes are co-expressed in the skeletal musculature and knockdown of Hsp90a leads to impaired myofibril formation in the same manner as lack of Steif/Unc-45b activity. Transcripts of both genes are up-regulated in steif mutants suggesting co-regulation of the two genes. Our data indicate a requirement of Steif/unc-45b and Hsp90a for the assembly of the contractile apparatus in the vertebrate skeletal musculature.
Links	PubMed Online version:10.1016/j.ydbio.2007.05.014
Keywords	Amino Acid Sequence; Animals; Animals, Genetically Modified; Base Sequence; DNA/genetics; Gene Expression Regulation, Developmental; HSP90 Heat-Shock Proteins/genetics; HSP90 Heat-Shock Proteins/metabolism; Heart/embryology; Microscopy, Electron, Transmission; Molecular Sequence Data; Muscle Development/genetics; Muscle Development/physiology; Muscle Proteins/genetics; Muscle Proteins/metabolism; Muscle, Skeletal/embryology; Muscle, Skeletal/metabolism; Muscle, Skeletal/ultrastructure; Mutation; Myocardium/metabolism; Myocardium/ultrastructure; Phenotype; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism
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