PMID:17560744

Citation	Pei, Y, Nicholson, V, Woods, K and Prescott, JF (2007) Immunization by intrabronchial administration to 1-week-old foals of an unmarked double gene disruption strain of Rhodococcus equi strain 103+. Vet. Microbiol. 125:100-10
Abstract	Rhodococcus equi causes fatal granulomatous pneumonia in foals and immunocompromised animals and humans. However, there is no effective vaccine against this infection. In this study, the chromosomal genes isocitrate lyase (icl) and cholesterol oxidase (choE) were chosen as targets for mutation and assessment of the double mutant as an intrabronchial vaccine in 1-week-old foals. Using a modification of a suicide plasmid previously developed in this laboratory, we developed a choE-icl unmarked deletion mutant of R. equi strain 103+. Five 1-week-old foals were infected intrabronchially with the mutant and challenged intrabronchially with the parent, virulent, strain 2 weeks later. Three of the foals were protected against pneumonia caused by the virulent strain, but the other two foals developed pneumonia caused by the mutant strain during the post-challenge period. Since infection of 3-week-old foals by an icl mutant in an earlier study had shown complete attenuation of the strain, we conclude that a proportion of foals in the 1st week or so of life are predisposed to developing R. equi pneumonia because of an inability to mount an effective immune response. This has been suspected previously but this is the first time that this has been demonstrated experimentally.
Links	PubMed Online version:10.1016/j.vetmic.2007.05.007
Keywords	Actinomycetales Infections/immunology; Actinomycetales Infections/microbiology; Actinomycetales Infections/prevention & control; Actinomycetales Infections/veterinary; Animals; Animals, Newborn; Bacterial Vaccines/administration & dosage; Bacterial Vaccines/genetics; Bacterial Vaccines/immunology; Cholesterol Oxidase/genetics; DNA, Bacterial/genetics; Female; Horse Diseases/immunology; Horse Diseases/microbiology; Horse Diseases/prevention & control; Horses; Immunization/methods; Immunization/veterinary; Isocitrate Lyase/genetics; Mice; Plasmids/genetics; Pneumonia, Bacterial/immunology; Pneumonia, Bacterial/microbiology; Pneumonia, Bacterial/prevention & control; Pneumonia, Bacterial/veterinary; Rhodococcus equi/genetics; Rhodococcus equi/immunology; Sequence Deletion/immunology; Vaccines, Attenuated/administration & dosage; Vaccines, Attenuated/genetics; Vaccines, Attenuated/immunology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BREST:CHOD		GO:0055114: oxidation-reduction process	ECO:0000315:		P		figure 1 choE is synonym of choB	complete CACAO 4926

References

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