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PMID:1753946
| Citation |
Ozier-Kalogeropoulos, O, Fasiolo, F, Adeline, MT, Collin, J and Lacroute, F (1991) Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase. Mol. Gen. Genet. 231:7-16 |
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| Abstract |
The URA7 gene of Saccharomyces cerevisiae encodes CTP synthetase (EC 6.3.4.2) which catalyses the conversion of uridine 5'-triphosphate to cytidine 5'-triphosphate, the last step of the pyrimidine biosynthetic pathway. We have cloned and sequenced the URA7 gene. The coding region is 1710 bp long and the deduced protein sequence shows a strong degree of homology with bacterial and human CTP synthetases. Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast. This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Blotting, Northern; Blotting, Southern; Carbon-Nitrogen Ligases; Chromatography, High Pressure Liquid; Cloning, Molecular; Fungal Proteins/genetics; Ligases/genetics; Ligases/metabolism; Molecular Sequence Data; Restriction Mapping; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Sequence Alignment |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
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