PMID:17535816

Citation	Jerga, A, Lu, YJ, Schujman, GE, de Mendoza, D and Rock, CO (2007) Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis. J. Biol. Chem. 282:21738-45
Abstract	Diacylglycerol kinases (DagKs) are key enzymes in lipid metabolism that function to reintroduce diacylglycerol formed from the hydrolysis of phospholipids into the biosynthetic pathway. Bacillus subtilis is a prototypical Gram-positive bacterium with a lipoteichoic acid structure containing repeating units of sn-glycerol-1-P groups derived from phosphatidylglycerol head groups. The B. subtilis homolog of the prokaryotic DagK gene family (dgkA; Pfam01219) was not a DagK but rather was an undecaprenol kinase. The three members of the soluble DagK protein family (Pfam00781) in B. subtilis were tested by complementation of an E. coli dgkA mutant, and only the essential yerQ gene possessed DagK activity. This gene was dubbed dgkB, and the soluble protein product was purified, and its DagK activity was verified in vitro. Conditional inactivation of dgkB led to the accumulation of diacylglycerol and the cessation of lipoteichoic acid formation in B. subtilis. This study identifies a soluble protein encoded by the dgkB (yerQ) gene as an essential kinase in the diacylglycerol cycle that drives lipoteichoic acid production.
Links	PubMed Online version:10.1074/jbc.M703536200
Keywords	Amino Acid Sequence; Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Bacterial Proteins/metabolism; DNA Primers; Diacylglycerol Kinase/deficiency; Diacylglycerol Kinase/genetics; Diacylglycerol Kinase/isolation & purification; Diacylglycerol Kinase/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Genetic Complementation Test; Kinetics; Lipopolysaccharides/biosynthesis; Molecular Sequence Data; Multigene Family; Plasmids; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Teichoic Acids/biosynthesis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:DAGK	enables	GO:0004143: diacylglycerol kinase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
BACSU:DAGK		GO:0004143: diacylglycerol kinase activity	ECO:0000314:		F		Figure 4 B,C show Dgkb kinase activity	complete CACAO 2135

References

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