PMID:17521365

Citation	Li, Y, Xia, H, Bai, F, Xu, H, Yang, L, Yao, H, Zhang, L, Zhang, X, Bai, Y, Saris, PE, Tolker-Nielsen, T and Qiao, M (2007) Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa. FEMS Microbiol. Lett. 272:188-95
Abstract	Flagella-mediated motility is recognized as one of the major factors contributing to virulence in Pseudomonas aeruginosa. During a screening of a mini-Mu transposon mutant library of P. aeruginosa PA68, a mutant partially deficient in swimming and swarming motility was identified in a new locus that encodes a predicted protein of unknown function annotated PA5017 in the P. aeruginosa PAO1 genome sequence. Chemotaxis plate assay indicated that inactivation of the PA5017 gene led to a decreased chemotactic response. Complementation of the PA5017 mutant with the wild-type PA5017 gene restored normal motility and chemotaxis phenotype. A promoter-lacZ reporter activity assay of the cheYZAB operon from chemotaxis gene cluster 1 showed that there was almost a twofold difference in expression levels of the wild-type PA68 and the PA5017 mutant. This suggested that the PA5017 affected expression of the cheYZAB operon negatively. Further study showed that inactivation of the PA5017 gene in PA68 led to increased biofilm formation in a static system and to the formation of a heterogeneous biofilm in a flow-chamber system. These results suggested that PA5017 possibly affected flagellum-dependent motility and in turn biofilm formation via the chemotaxis signal transduction pathway.
Links	PubMed Online version:10.1111/j.1574-6968.2007.00753.x
Keywords	Artificial Gene Fusion; Biofilms; Chemotaxis/genetics; DNA Transposable Elements; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Flagella/genetics; Flagella/ultrastructure; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Reporter; Genetic Complementation Test; Locomotion/genetics; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Molecular Sequence Data; Mutagenesis, Insertional; Operon; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/physiology; Sequence Analysis, DNA; beta-Galactosidase/analysis; beta-Galactosidase/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PSEAE:Q9HUF2		GO:0071977: bacterial-type flagellar swimming motility	ECO:0000315:		P		As seen in figure 1, part a through c, the PA5017 mutant displayed reduced swimming in medium containing 0.2% agar. The ability to swim decreased further as the concentration of agar increased and was lost in medium containing more than 0.3% agar. These results indicated that PA5017 was involved in flagellum-mediated motility. Figure 2 shows flagellum.	complete CACAO 4844
PSEAE:Q9HUF2		GO:0071978 : bacterial-type flagellar swarming motility	ECO:0000315:		P		As seen in figure 1, parts d and e, in the swarming assay, the wild-type PA68 strain exhibited a branching growth pattern away from the point of inoculation under all tested agar concentrations, while the PA5017 mutant displaced reduced swarming motility at a relatively low agar concentration (0.45%), and could not swarm at higher agar concentrations. These results indicated that PA5017 was involved in flagellum-mediated motility. Figure 2 shows flagellum.	complete CACAO 4845
PSEAE:Q9HUF2		GO:0006935: chemotaxis	ECO:0000315:		P		Chemotaxis behavior was tested using casamino acids as the chemoattractant. As seen in figure 1 part f, the mutant showed a decreased response to chemoattractant compared with the wild type, suggesting that the PA5017 gene was involved (directly or indirectly) in chemotaxis.	complete CACAO 4846

References

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