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PMID:17504936
| Citation |
King, EM, Rachidi, N, Morrice, N, Hardwick, KG and Stark, MJ (2007) Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores. Genes Dev. 21:1163-8 |
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| Abstract |
The spindle checkpoint delays anaphase onset until all chromosomes are correctly attached to microtubules. Ipl1 protein kinase (Aurora B) is required to correct inappropriate kinetochore-microtubule attachments and for the response to lack of tension between sister kinetochores. Here we identify residues in the checkpoint protein Mad3p that are phosphorylated by Ipl1p. When phosphorylation of Mad3p at two sites is prevented, the cell's response to reduced kinetochore tension is dramatically curtailed. Our data provide strong evidence for a distinct checkpoint pathway responding to lack of sister kinetochore tension, in which Ipl1p-dependent phosphorylation of Mad3p is a key step. |
| Links |
PubMed PMC1865488 Online version:10.1101/gad.431507 |
| Keywords |
Blotting, Western; Cell Cycle Proteins/metabolism; Electrophoresis, Polyacrylamide Gel; Genes, cdc/physiology; Immunohistochemistry; Kinetochores/metabolism; Mitotic Spindle Apparatus/metabolism; Nuclear Proteins/metabolism; Protein-Serine-Threonine Kinases/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Yeasts |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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