GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:17464079
| Citation |
Xu, Z, Tian, B, Sun, Z, Lin, J and Hua, Y (2007) Identification and functional analysis of a phytoene desaturase gene from the extremely radioresistant bacterium Deinococcus radiodurans. Microbiology (Reading, Engl.) 153:1642-52 |
|---|---|
| Abstract |
The phytoene-related desaturases are the key enzymes in the carotenoid biosynthetic pathway. The gene encoding phytoene desaturase in the deinoxanthin synthesis pathway of Deinococcus radiodurans was identified and characterized. Two putative phytoene desaturase homologues (DR0861 and DR0810) were identified by analysis of conserved amino acid regions, and the former displayed the highest identity (68 %) with phytoene desaturase of the cyanobacterium Gloeobacter violaceus. DR0861 gene knockout and dinucleotide-binding motif deletion resulted in the arrest of lycopene synthesis and the accumulation of phytoene. The colourless DR0861 knockout mutant became more sensitive to acute ionizing radiation and oxygen stress. Complementation of the mutant with a heterologous or homologous gene restored its pigment and resistance. The desaturase activity of DR0861 (crtI) was further confirmed by the assay of enzyme activity in vitro and heterologous expression in Escherichia coli containing crtE and crtB genes (responsible for phytoene synthesis) from Erwinia uredovora. In addition, the amount of lycopene synthesis in E. coli resulting from the expression of crtI from D. radiodurans was determined, and this had significant dose-dependent effects on the survival rate of E. coli exposed to hydrogen peroxide and ionizing radiation. |
| Links |
PubMed Online version:10.1099/mic.0.2006/002857-0 |
| Keywords |
Amino Acid Sequence; Anti-Bacterial Agents/pharmacology; Carotenoids/biosynthesis; Computational Biology; Conserved Sequence; Cyanobacteria/genetics; Deinococcus/drug effects; Deinococcus/enzymology; Deinococcus/genetics; Deinococcus/radiation effects; Erwinia/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/radiation effects; Gene Deletion; Genetic Complementation Test; Molecular Sequence Data; Mutagenesis, Insertional; Oxidants/pharmacology; Oxidoreductases/genetics; Oxidoreductases/metabolism; Pigments, Biological/biosynthesis; Sequence Homology, Amino Acid; Ultraviolet Rays |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016491: oxidoreductase activity |
ECO:0000314: |
F |
Figure 5: Purified DR0861 protein from D. radioduran converted 15,15'-cis-phytoene to lycopene detected by HPLC. This confirmed that the lycopene synthesized from phytoene in D. radioduran was catalyzed by the DR0681 protein. |
complete | ||||
|
enables |
GO:0016491: oxidoreductase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
