GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17409115

From GONUTS
Jump to: navigation, search
Citation

Lee, S, Nahm, M, Lee, M, Kwon, M, Kim, E, Zadeh, AD, Cao, H, Kim, HJ, Lee, ZH, Oh, SB, Yim, J, Kolodziej, PA and Lee, S (2007) The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion. Development 134:1767-77

Abstract

Axon extension and guidance require a coordinated assembly of F-actin and microtubules as well as regulated translation. The molecular basis of how the translation of mRNAs encoding guidance proteins could be closely tied to the pace of cytoskeletal assembly is poorly understood. Previous studies have shown that the F-actin-microtubule crosslinker Short stop (Shot) is required for motor and sensory axon extension in the Drosophila embryo. Here, we provide biochemical and genetic evidence that Shot functions with a novel translation inhibitor, Krasavietz (Kra, Exba), to steer longitudinally directed CNS axons away from the midline. Kra binds directly to the C-terminus of Shot, and this interaction is required for the activity of Shot to support midline axon repulsion. shot and kra mutations lead to weak robo-like phenotypes, and synergistically affect midline avoidance of CNS axons. We also show that shot and kra dominantly enhance the frequency of midline crossovers in embryos heterozygous for slit or robo, and that in kra mutant embryos, some Robo-positive axons ectopically cross the midline that normally expresses the repellent Slit. Finally, we demonstrate that Kra also interacts with the translation initiation factor eIF2beta and inhibits translation in vitro. Together, these data suggest that Kra-mediated translational regulation plays important roles in midline axon repulsion and that Shot functions as a direct physical link between translational regulation and cytoskeleton reorganization.

Links

PubMed Online version:10.1242/dev.02842

Keywords

Amino Acid Sequence; Animals; Axons/physiology; Central Nervous System/embryology; Central Nervous System/metabolism; Central Nervous System/virology; Drosophila/embryology; Drosophila/metabolism; Drosophila Proteins/chemistry; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Embryo, Nonmammalian/metabolism; Eukaryotic Initiation Factor-5/genetics; Eukaryotic Initiation Factor-5/metabolism; Eukaryotic Initiation Factors/metabolism; Gene Expression Regulation, Developmental; Microfilament Proteins/chemistry; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Microtubules; Molecular Sequence Data; Nerve Tissue Proteins/metabolism; Neurons/metabolism; Protein Biosynthesis; Protein Structure, Tertiary; Receptors, Immunologic/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.