PMID:17349656

Citation	Chow, AK, Cena, J, El-Yazbi, AF, Crawford, BD, Holt, A, Cho, WJ, Daniel, EE and Schulz, R (2007) Caveolin-1 inhibits matrix metalloproteinase-2 activity in the heart. J. Mol. Cell. Cardiol. 42:896-901
Abstract	Apart from its ability to degrade extracellular matrix proteins, matrix metalloproteinase-2 (MMP-2) was recently revealed to have targets and actions within the cardiac myocyte. The localization of MMP-2 in caveolae of endothelial cells suggests that caveolin-1 (Cav-1) may play a role in regulating MMP-2. The caveolin scaffolding domain (CSD) of Cav-1 regulates several proteins including those involved with signaling cascades. Whether Cav-1 is responsible for regulating MMP-2 in the heart is unknown. Hearts from Cav-1(-/-) or Cav-1(+/+) mice were isolated and heart extracts or lipid raft enriched membrane fractions were prepared. MMP-2 activity in Cav-1(-/-) hearts was markedly enhanced when compared with Cav-1(+/+) hearts with no changes in MMP-2 protein levels between groups. In contrast, MMP-2 activity and protein level were greatly reduced in lipid raft enriched fractions of Cav-1(-/-) hearts. Purified CSD inhibited MMP-2 activity in a concentration-dependent manner as assessed using an in vitro degradation assay with a fluorogenic MMP-2 substrate (OmniMMP). These data suggest that Cav-1 plays a role in regulating MMP-2 activity. Cav-1 may thus be a novel mechanism to regulate MMP-2 activity in the heart.
Links	PubMed Online version:10.1016/j.yjmcc.2007.01.008
Keywords	Amino Acid Sequence; Animals; Caveolin 1/genetics; Caveolin 1/physiology; Heart/physiology; Male; Matrix Metalloproteinase 2/antagonists & inhibitors; Matrix Metalloproteinase 2/metabolism; Mice; Mice, Knockout; Molecular Sequence Data; Myocytes, Cardiac/metabolism
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