PMID:17322317

Citation	Puri-Taneja, A, Schau, M, Chen, Y and Hulett, FM (2007) Regulators of the Bacillus subtilis cydABCD operon: identification of a negative regulator, CcpA, and a positive regulator, ResD. J. Bacteriol. 189:3348-58
Abstract	The cydABCD operon of Bacillus subtilis encodes products required for the production of cytochrome bd oxidase. Previous work has shown that one regulatory protein, YdiH (Rex), is involved in the repression of this operon. The work reported here confirms the role of Rex in the negative regulation of the cydABCD operon. Two additional regulatory proteins for the cydABCD operon were identified, namely, ResD, a response regulator involved in the regulation of respiration genes, and CcpA, the carbon catabolite regulator protein. ResD, but not ResE, was required for full expression of the cydA promoter in vivo. ResD binding to the cydA promoter between positions -58 and -107, a region which includes ResD consensus binding sequences, was not enhanced by phosphorylation. A ccpA mutant had increased expression from the full-length cydA promoter during stationary growth compared to the wild-type strain. Maximal expression in a ccpA mutant was observed from a 3'-deleted cydA promoter fusion that lacked the Rex binding region, suggesting that the effect of the two repressors, Rex and CcpA, was cumulative. CcpA binds directly to the cydA promoter, protecting the region from positions -4 to -33, which contains sequences similar to the CcpA consensus binding sequence, the cre box. CcpA binding was enhanced upon addition of glucose-6-phosphate, a putative cofactor for CcpA. Mutation of a conserved residue in the cre box reduced CcpA binding 10-fold in vitro and increased cydA expression in vivo. Thus, CcpA and ResD, along with the previously identified cydA regulator Rex (YdiH), affect the expression of the cydABCD operon. Low-level induction of the cydA promoter was observed in vivo in the absence of its regulatory proteins, Rex, CcpA, and ResD. This complex regulation suggests that the cydA promoter is tightly regulated to allow its expression only at the appropriate time and under the appropriate conditions.
Links	PubMed PMC1855890 Online version:10.1128/JB.00050-07
Keywords	Artificial Gene Fusion; Bacillus subtilis/genetics; Bacillus subtilis/physiology; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Base Sequence; Cytochromes/biosynthesis; DNA Footprinting; DNA Transposable Elements; DNA, Bacterial/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Electrophoretic Mobility Shift Assay; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Regulator; Genes, Reporter; Molecular Sequence Data; Mutagenesis, Insertional; Operon; Protein Binding; Repressor Proteins/genetics; Repressor Proteins/physiology; Transcription Factors/genetics; Transcription Factors/physiology; beta-Galactosidase/biosynthesis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:RESD		GO:0010628: positive regulation of gene expression	ECO:0000315:		P		FIG. 3. cydA expression requires ResD but not ResE. (A) In vivo expression of full-length cydABCD promoter. (B) In vivo expression of cydABCD promoter lacking Rex binding sites. Growth and β-galactosidase expression in cells cultured for 8 h in NSMPG are shown.	complete

References

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