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PMID:1729652
| Citation |
Franzusoff, A, Lauzé, E and Howell, KE (1992) Immuno-isolation of Sec7p-coated transport vesicles from the yeast secretory pathway. Nature 355:173-5 |
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| Abstract |
The transport of proteins destined for post-endoplasmic reticulum locations in the secretory pathway is mediated by small vesicular carriers. Transport vesicles have been generated in cell-free assays from the yeast Saccharomyces cerevisiae, and mammalian systems. Yeast genes encoding cytosolic components that participate in vesicular traffic were first identified from the collection of conditional-lethal sec-(secretory) mutants. Mutations in the yeast SEC7 gene disrupt protein transport in the secretory pathway at the nonpermissive temperature. The SEC7 gene product is a phosphoprotein of relative molecular mass 230,000 that functions from the cytoplasmic aspect of intracellular membranes. We report that in a yeast cell-free transport assay, the introduction of antibodies to Sec7 protein (Sec7p) results in the accumulation of transport vesicles. These vesicles are retrieved with Sec7p-specific antibodies by immuno-isolation for biochemical and electron microscopic characterization. Sec7p on the surface of the accumulated transport vesicles, in combination with previous genetic and biochemical studies, implicate Sec7p as part of a (non-clathrin) vesicle coat. This Sec7p-containing coat structure is proposed to be essential for vesicle budding at multiple stages in the yeast secretory pathway. |
| Links |
PubMed Online version:10.1038/355173a0 |
| Keywords |
Biological Transport; Cell Fractionation; Endoplasmic Reticulum/chemistry; Fungal Proteins/analysis; Fungal Proteins/immunology; Fungal Proteins/secretion; Golgi Apparatus/chemistry; Guanine Nucleotide Exchange Factors; Immunosorbent Techniques; Magnetics; Organelles/chemistry; Organelles/metabolism; Organelles/ultrastructure; Peptides/metabolism; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/ultrastructure |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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