PMID:17287248

Citation	Liu, Y, Pathak, N, Kramer-Zucker, A and Drummond, IA (2007) Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Development 134:1111-22
Abstract	Epithelial tubules consist of multiple cell types that are specialized for specific aspects of organ function. In the zebrafish pronephros, multiciliated cells (MCCs) are specialized for fluid propulsion, whereas transporting epithelial cells recover filtered-blood solutes. These cell types are distributed in a ;salt-and-pepper' fashion in the pronephros, suggesting that a lateral inhibition mechanism may play a role in their differentiation. We find that the Notch ligand Jagged 2 is expressed in MCCs and that notch3 is expressed in pronephric epithelial cells. Morpholino knockdown of either jagged 2 or notch3, or mutation in mind bomb (in which Notch signaling is impaired), dramatically expands ciliogenic gene expression, whereas ion transporter expression is lost, indicating that pronephric cells are transfated to MCCs. Conversely, ectopic expression of the Notch1a intracellular domain represses MCC differentiation. Gamma-secretase inhibition using DAPT demonstrated a requirement for Notch signaling early in pronephric development, before the pattern of MCC differentiation is apparent. Strikingly, we find that jagged 2 knockdown generates extra cilia and is sufficient to rescue the kidney cilia mutant double bubble. Our results indicate that Jagged 2/Notch signaling modulates the number of multiciliated versus transporting epithelial cells in the pronephros by way of a genetic pathway involving repression of rfx2, a key transcriptional regulator of the ciliogenesis program.
Links	PubMed Online version:10.1242/dev.02806
Keywords	Amyloid Precursor Protein Secretases/antagonists & inhibitors; Amyloid Precursor Protein Secretases/metabolism; Animals; Calcium-Binding Proteins/antagonists & inhibitors; Calcium-Binding Proteins/genetics; Calcium-Binding Proteins/metabolism; Cell Differentiation; Cilia/metabolism; Cilia/physiology; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Epithelium/metabolism; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Nephrons/cytology; Nephrons/embryology; Nephrons/metabolism; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Receptor, Notch1/genetics; Receptor, Notch1/metabolism; Receptors, Notch/genetics; Receptors, Notch/physiology; Signal Transduction; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins/antagonists & inhibitors; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism; Zebrafish Proteins/physiology
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.