PMID:17286805

Citation	Gardiner, FC, Costa, R and Ayscough, KR (2007) Nucleocytoplasmic trafficking is required for functioning of the adaptor protein Sla1p in endocytosis. Traffic 8:347-58
Abstract	Dual localization of proteins at the plasma membrane and within the nucleus has been reported in mammalian cells. Among these proteins are those involved in cell adhesion structures and in clathrin-mediated endocytosis. In the case of endocytic proteins, trafficking to the nucleus is not known to play a role in their endocytic function. Here, we show localization of the yeast endocytic adaptor protein Sla1p to the nucleus as well as to the cell cortex and we demonstrate the importance of specific regions of Sla1p for this nuclear localization. A role for specific karyopherins (importins and exportins) in Sla1p nuclear localization is revealed. Furthermore, endocytosis of Sla1p-dependent cargo is defective in three strains with karyopherin mutations. Finally, we investigate possible functions for nuclear trafficking of endocytic proteins. Our data reveal for the first time that nuclear transport of endocytic proteins is important for functional endocytosis in Saccharomyces cerevisiae. We determine the mechanism, involving an alpha/beta importin pair, that facilitates uptake of Sla1p and demonstrate that nuclear transport is required for the functioning of Sla1p during endocytosis.
Links	PubMed PMC1989034 Online version:10.1111/j.1600-0854.2007.00534.x
Keywords	Active Transport, Cell Nucleus/physiology; Carrier Proteins/metabolism; Carrier Proteins/physiology; Cell Nucleus/metabolism; Cell Nucleus/physiology; Cytoplasm/metabolism; Cytoplasm/physiology; Cytoskeletal Proteins; Endocytosis/physiology; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology
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