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PMID:17284516
| Citation |
Naetar, N, Hutter, S, Dorner, D, Dechat, T, Korbei, B, Gotzmann, J, Beug, H and Foisner, R (2007) LAP2alpha-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit. J. Cell. Sci. 120:737-47 |
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| Abstract |
Lamina-associated polypeptide 2alpha (LAP2alpha) is a nuclear protein dynamically associating with chromatin during the cell cycle. In addition, LAP2alpha interacts with A-type lamins and retinoblastoma protein and regulates cell cycle progression via the E2F-Rb pathway. Using yeast two-hybrid analysis and three independent in vitro binding assays we identified a new LAP2alpha interaction partner of hitherto unknown functions, which we termed LINT-25. LINT-25 protein levels were upregulated during G1 phase in proliferating cells and upon cell cycle exit in quiescence, senescence and differentiation. Upon cell cycle exit LINT-25 accumulated in heterochromatin foci, and LAP2alpha protein levels were downregulated by proteasomal degradation. Although LAP2alpha was not required for the upregulation and reorganization of LINT-25 during cell cycle exit, transient expression of LINT-25 in proliferating cells caused loss of LAP2alpha and subsequent cell death. Our data show a role of LINT-25 and LAP2alpha during cell cycle exit, in which LINT-25 acts upstream of LAP2alpha. |
| Links |
PubMed Online version:10.1242/jcs.03390 |
| Keywords |
Animals; Cell Cycle/genetics; Cell Cycle/physiology; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Differentiation/drug effects; Cell Differentiation/physiology; Cell Line; Cells, Cultured; Chromatin/metabolism; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Gene Expression Regulation; HeLa Cells; Humans; Immunoblotting; Immunoprecipitation; Lamins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Microscopy, Fluorescence; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Transfection; Two-Hybrid System Techniques |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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