PMID:17239364

Citation	Walker, MB, Miller, CT, Swartz, ME, Eberhart, JK and Kimmel, CB (2007) phospholipase C, beta 3 is required for Endothelin1 regulation of pharyngeal arch patterning in zebrafish. Dev. Biol. 304:194-207
Abstract	Genetic and pharmacological studies demonstrate that Endothelin1 (Edn1) is a key signaling molecule for patterning the facial skeleton in fish, chicks, and mice. When Edn1 function is reduced early in development the ventral lower jaw and supporting structures are reduced in size and often fused to their dorsal upper jaw counterparts. We show that schmerle (she) encodes a zebrafish ortholog of Phospholipase C, beta 3 (Plcbeta3) required in cranial neural crest cells for Edn1 regulation of pharyngeal arch patterning. Sequencing and co-segregation demonstrates that two independent she (plcbeta3) alleles have missense mutations in conserved residues within the catalytic domains of Plcbeta3. Homozygous plcbeta3 mutants are phenotypically similar to edn1 mutants and exhibit a strong arch expression defect in Edn1-dependent Distalless (Dlx) genes as well as expression defects in several Edn1-dependent intermediate and ventral arch domain transcription factors. plcbeta3 also genetically interacts with edn1, supporting a model in which Edn1 signals through a G protein-coupled receptor to activate Plcbeta3. Mild skeletal defects occur in plcbeta3 heterozygotes, showing the plcbeta3 mutations are partially dominant. Through a morpholino-mediated deletion in the N-terminal PH domain of Plcbeta3, we observe a partial rescue of facial skeletal defects in homozygous plcbeta3 mutants, supporting a hypothesis that an intact PH domain is necessary for the partial dominance we observe. In addition, through mosaic analyses, we show that wild-type neural crest cells can efficiently rescue facial skeletal defects in homozygous plcbeta3 mutants, demonstrating that Plcbeta3 function is required in neural crest cells and not other cell types to pattern the facial skeleton.
Links	PubMed PMC1906931 Online version:10.1016/j.ydbio.2006.12.027
Keywords	Animals; Base Sequence; Body Patterning/physiology; Branchial Region/embryology; Branchial Region/enzymology; DNA Primers; Endothelin-1/metabolism; Gene Expression Regulation, Developmental; Isoenzymes/genetics; Isoenzymes/metabolism; Microscopy, Confocal; Molecular Sequence Data; Mutation, Missense/genetics; Neural Crest/embryology; Neural Crest/metabolism; Phospholipase C beta; Sequence Analysis, DNA; Signal Transduction/genetics; Type C Phospholipases/genetics; Type C Phospholipases/metabolism; Zebrafish/embryology; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.