PMID:17203076

Citation	Tsang, CK, Li, H and Zheng, XS (2007) Nutrient starvation promotes condensin loading to maintain rDNA stability. EMBO J. 26:448-58
Abstract	Nutrient starvation or rapamycin treatment, through inhibition of target of rapamycin, causes condensation of ribosomal DNA (rDNA) array and nucleolar contraction in budding yeast. Here we report that under such conditions, condensin is rapidly relocated into the nucleolus and loaded to rDNA tandem repeats, which is required for rDNA condensation. Rpd3-dependent histone deacetylation is necessary and sufficient for condensin's relocalization and loading to rDNA array, suggesting that histone modification plays a regulatory role for condensin targeting. Rapamycin independently, yet coordinately, inhibits rDNA transcription and promotes condensin loading to rDNA array. Unexpectedly, we found that inhibition of rDNA transcription in the absence of condensin loading leads to rDNA instability. Our data suggest that enrichment of condensin prevents rDNA instability during nutrient starvation. Together, these observations unravel a novel role for condensin in the maintenance of regional genomic stability.
Links	PubMed PMC1783468 Online version:10.1038/sj.emboj.7601488
Keywords	Adenosine Triphosphatases/metabolism; Adenosine Triphosphatases/physiology; Antifungal Agents/pharmacology; Cell Nucleolus/drug effects; Cell Nucleolus/metabolism; Chromosome Segregation; Chromosomes, Fungal/metabolism; DNA Packaging; DNA, Ribosomal/drug effects; DNA, Ribosomal/metabolism; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Food; Genomic Instability; Histone Deacetylases/physiology; Multiprotein Complexes/metabolism; Multiprotein Complexes/physiology; Organisms, Genetically Modified; Saccharomycetales; Sirolimus/pharmacology; Transcription, Genetic/drug effects
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