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PMID:17202207
| Citation |
Karhu, NJ, Ziedaite, G, Bamford, DH and Bamford, JK (2007) Efficient DNA packaging of bacteriophage PRD1 requires the unique vertex protein P6. J. Virol. 81:2970-9 |
|---|---|
| Abstract |
The assembly of bacteriophage PRD1 proceeds via formation of empty procapsids containing an internal lipid membrane, into which the linear double-stranded DNA genome is subsequently packaged. The packaging ATPase P9 and other putative packaging proteins have been shown to be located at a unique vertex of the PRD1 capsid. Here, we describe the isolation and characterization of a suppressor-sensitive PRD1 mutant deficient in the unique vertex protein P6. Protein P6 was found to be an essential part of the PRD1 packaging machinery; its absence leads to greatly reduced packaging efficiency. Lack of P6 was not found to affect particle assembly, because in the P6-deficient mutant infection, wild-type (wt) amounts of particles were produced, although most were empty. P6 was determined not to be a specificity factor, as the few filled particles seen in the P6-deficient infection contained only PRD1-specific DNA. The presence of P6 was not necessary for retention of DNA in the capsid once packaging had occurred, and P6-deficient DNA-containing particles were found to be stable and infectious, albeit not as infectious as wt PRD1 virions. A packaging model for bacteriophage PRD1, based on previous results and those obtained in this study, is presented. |
| Links |
PubMed PMC1865968 Online version:10.1128/JVI.02211-06 |
| Keywords |
Bacteriophage PRD1/genetics; Bacteriophage PRD1/metabolism; Bacteriophage PRD1/ultrastructure; DNA Packaging; Mutation; Salmonella enterica/ultrastructure; Salmonella enterica/virology; Viral Proteins/genetics; Viral Proteins/metabolism; Virion/isolation & purification; Virion/metabolism; Virion/ultrastructure; Virus Assembly |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019073: viral DNA genome packaging |
ECO:0000315: |
P |
Figure 2. |
complete | ||||
| GO:0019012: virion |
ECO:0000315: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019073: viral DNA genome packaging |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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