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PMID:17190806
| Citation |
Straight, PD, Fischbach, MA, Walsh, CT, Rudner, DZ and Kolter, R (2007) A singular enzymatic megacomplex from Bacillus subtilis. Proc. Natl. Acad. Sci. U.S.A. 104:305-10 |
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| Abstract |
Nonribosomal peptide synthetases (NRPS), polyketide synthases (PKS), and hybrid NRPS/PKS are of particular interest, because they produce numerous therapeutic agents, have great potential for engineering novel compounds, and are the largest enzymes known. The predicted masses of known enzymatic assembly lines can reach almost 5 megadaltons, dwarfing even the ribosome (approximately 2.6 megadaltons). Despite their uniqueness and importance, little is known about the organization of these enzymes within the native producer cells. Here we report that an 80-kb gene cluster, which occupies approximately 2% of the Bacillus subtilis genome, encodes the subunits of approximately 2.5 megadalton active hybrid NRPS/PKS. Many copies of the NRPS/PKS assemble into a single organelle-like membrane-associated complex of tens to hundreds of megadaltons. Such an enzymatic megacomplex is unprecedented in bacterial subcellular organization and has important implications for engineering novel NRPS/PKSs. |
| Links |
PubMed PMC1765455 Online version:10.1073/pnas.0609073103 |
| Keywords |
Amino Acid Sequence; Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Cryoelectron Microscopy; Molecular Sequence Data; Multigene Family; Peptide Synthases/genetics; Polyketide Synthases/genetics; Protein Engineering |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0034629: cellular protein complex localization |
IDA: Inferred from Direct Assay: |
P |
Figure 2B This is a cyroelectron experiment showing that both pksR and pksE are localizing in the same spot, to form a protein complex in the cell.(megacomplex). These are not mutants, they are just tagged with a fluorescent gene. |
complete |
| |||
| GO:0034629: cellular protein complex localization |
IDA: Inferred from Direct Assay: |
P |
Figure 2B This is a cryoelectron microscopy experiment that shows pksR and pksE localize to the same spot to form a protein complex(megacomplex) in the cell |
complete |
| |||
Notes
See also
References
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