PMID:17134691

Citation	Nie, S and Chang, C (2007) Regulation of Xenopus gastrulation by ErbB signaling. Dev. Biol. 303:93-107
Abstract	During Xenopus gastrulation, mesendodermal cells are internalized and display different movements. Head mesoderm migrates along the blastocoel roof, while trunk mesoderm undergoes convergent extension (C&E). Different signals are implicated in these processes. Our previous studies reveal that signals through ErbB receptor tyrosine kinases modulate Xenopus gastrulation, but the mechanisms employed are not understood. Here we report that ErbB signals control both C&E and head mesoderm migration. Inhibition of ErbB pathway blocks elongation of dorsal marginal zone explants and activin-treated animal caps without removing mesodermal gene expression. Bipolar cell shape and cell mixing in the dorsal region are impaired. Inhibition of ErbB signaling also interferes with migration of prechordal mesoderm on fibronectin. Cell-cell and cell-matrix interaction and cell spreading are reduced when ErbB signaling is blocked. Using antisense morpholino oligonucleotides, we show that ErbB4 is involved in Xenopus gastrulation morphogenesis, and it partially regulates cell movements through modulation of cell adhesion and membrane protrusions. Our results reveal for the first time that vertebrate ErbB signaling modulates gastrulation movements, thus providing a novel pathway, in addition to non-canonical Wnt and FGF signals, that controls gastrulation. We further demonstrate that regulation of cell adhesive properties and cell morphology may underlie the functions of ErbBs in gastrulation.
Links	PubMed Online version:10.1016/j.ydbio.2006.10.039
Keywords	Animals; Cell Adhesion/physiology; Cell Movement/physiology; Gastrula/physiology; Immunohistochemistry; In Situ Hybridization; In Situ Nick-End Labeling; Mesoderm/physiology; Morphogenesis/physiology; Oligonucleotides; Receptor, Epidermal Growth Factor/metabolism; Signal Transduction/physiology; Xenopus/embryology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
XENLA:Q153D9		GO:0060031: mediolateral intercalation	ECO:0000315:		P		Generated mutant ErbB4 receptors (truncated receptors) performed in vitro explant assay to understand if ErbB signaling is involved in regulation of head mesoderm migration (Figure 6)	complete CACAO 7173
XENLA:Q153D9	involved_in	GO:0060031: mediolateral intercalation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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