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PMID:17114060
| Citation |
Nievera, C, Torgue, JJ, Grimwade, JE and Leonard, AC (2006) SeqA blocking of DnaA-oriC interactions ensures staged assembly of the E. coli pre-RC. Mol. Cell 24:581-92 |
|---|---|
| Abstract |
DnaA occupies only the three highest-affinity binding sites in E. coli oriC throughout most of the cell cycle. Immediately prior to initiation of chromosome replication, DnaA interacts with additional recognition sites, resulting in localized DNA-strand separation. These two DnaA-oriC complexes formed during the cell cycle are functionally and temporally analogous to yeast ORC and pre-RC. After initiation, SeqA binds to hemimethylated oriC, sequestering oriC while levels of active DnaA are reduced, preventing reinitiation. In this paper, we investigate how resetting of oriC to the ORC-like complex is coordinated with SeqA-mediated sequestration. We report that oriC resets to ORC during sequestration. This was possible because SeqA blocked DnaA binding to hemimethylated oriC only at low-affinity recognition sites associated with GATC but did not interfere with occupation of higher-affinity sites. Thus, during the sequestration period, SeqA repressed pre-RC assembly while ensuring resetting of E. coli ORC. |
| Links |
PubMed PMC1939805 Online version:10.1016/j.molcel.2006.09.016 |
| Keywords |
Bacterial Outer Membrane Proteins/physiology; Bacterial Proteins/metabolism; Base Sequence; DNA Footprinting; DNA Methylation; DNA Replication/physiology; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/physiology; Macromolecular Substances/metabolism; Molecular Sequence Data; Origin Recognition Complex/genetics; Origin Recognition Complex/metabolism; Protein Binding |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0032297: negative regulation of DNA-dependent DNA replication initiation |
ECO:0000314: |
P |
Figure 5 shows SeqA inhibits DnaA-induced DNA strand separation of hemimethylated supercoiled DNA |
complete | ||||
See also
References
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