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PMID:17085695
| Citation |
Sass, P and Bierbaum, G (2007) Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl. Environ. Microbiol. 73:347-52 |
|---|---|
| Abstract |
The recombinant phi11 endolysin hydrolyzed heat-killed staphylococci as well as staphylococcal biofilms. Cell wall targeting appeared to be a prerequisite for lysis of whole cells, and the combined action of the endopeptidase and amidase domains was necessary for maximum activity. In contrast, the phi12 endolysin was inactive and caused aggregation of the cells. |
| Links |
PubMed PMC1797112 Online version:10.1128/AEM.01616-06 |
| Keywords |
Amidohydrolases/metabolism; Bacteriolysis; Biofilms/drug effects; Biofilms/growth & development; Endopeptidases/genetics; Endopeptidases/metabolism; Endopeptidases/pharmacology; Hot Temperature; Lysogeny; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Recombinant Proteins/pharmacology; Staphylococcus Phages/genetics; Staphylococcus Phages/metabolism; Staphylococcus aureus/cytology; Staphylococcus aureus/drug effects; Staphylococcus aureus/genetics; Staphylococcus aureus/growth & development |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009253: peptidoglycan catabolic process |
ECO:0000314: |
P |
Figure 3 shows "The full-length enzyme showed efficient lysis of the peptidoglycan" from S.simulans. |
complete | ||||
| GO:0001897: cytolysis by symbiont of host cells |
ECO:0000314: |
P |
Figure 4 shows "The phi11 endolysin lyses whole cells of several staphylococci" |
complete | ||||
|
involved_in |
GO:0001897: cytolysis by symbiont of host cells |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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