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PMID:17074814

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Citation

Garbett, KA, Tripathi, MK, Cencki, B, Layer, JH and Weil, PA (2007) Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction. Mol. Cell. Biol. 27:297-311

Abstract

In vivo studies have previously shown that Saccharomyces cerevisiae ribosomal protein (RP) gene expression is controlled by the transcription factor repressor activator protein 1 (Rap1p) in a TFIID-dependent fashion. Here we have tested the hypothesis that yeast TFIID serves as a coactivator for RP gene transcription by directly interacting with Rap1p. We have found that purified recombinant Rap1p specifically interacts with purified TFIID in pull-down assays, and we have mapped the domains of Rap1p and subunits of TFIID responsible. In vitro transcription of a UAS(RAP1) enhancer-driven reporter gene requires both Rap1p and TFIID and is independent of the Fhl1p-Ifh1p coregulator. UAS(RAP1) enhancer-driven transactivation in extracts depleted of both Rap1p and TFIID is efficiently rescued by addition of physiological amounts of these two purified factors but not TATA-binding protein. We conclude that Rap1p and TFIID directly interact and that this interaction contributes importantly to RP gene transcription.

Links

PubMed PMC1800639 Online version:10.1128/MCB.01558-06

Keywords

Binding, Competitive; DNA-Binding Proteins/chemistry; Enhancer Elements, Genetic; Gene Expression Regulation, Fungal; Protein Binding; Protein Interaction Mapping; Protein Structure, Tertiary; Recombinant Proteins/chemistry; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; TATA-Box Binding Protein/chemistry; Telomere-Binding Proteins/chemistry; Telomere-Binding Proteins/metabolism; Telomere-Binding Proteins/physiology; Transcription Factor TFIID/chemistry; Transcription Factor TFIID/metabolism; Transcription Factors/chemistry; Transcription Factors/metabolism; Transcription Factors/physiology; Transcription, Genetic; Transcriptional Activation

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


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References

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