GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:17041587
| Citation |
Orme, MH, Alrubaie, S, Bradley, GL, Walker, CD and Leevers, SJ (2006) Input from Ras is required for maximal PI(3)K signalling in Drosophila. Nat. Cell Biol. 8:1298-302 |
|---|---|
| Abstract |
Class I phosphoinositide 3-kinases (PI(3)Ks) are activated through associated adaptor molecules in response to G protein-coupled and tyrosine kinase receptor signalling. They contain Ras-binding domains (RBDs) and can also be activated through direct association with active GTP-bound Ras. The ability of Ras to activate PI(3)K has been established in vitro and by overexpression analysis, but its relevance for normal PI(3)K function in vivo is unknown. The Drosophila class I PI(3)K, Dp110, is activated by nutrient-responsive insulin signalling and modulates growth, oogenesis and metabolism. To investigate the importance of Ras-mediated PI(3)K activation for normal PI(3)K function, we replaced Dp110 with Dp110(RBD), which is unable to bind to Ras but otherwise biochemically normal. We found that Ras-mediated Dp110 regulation is dispensable for viability. However, egg production, which requires large amounts of growth, is dramatically lowered in Dp110(RBD) flies. Furthermore, insulin cannot maximally activate PI(3)K signalling in Dp110(RBD) imaginal discs and Dp110(RBD) flies are small. Thus, Dp110 integrates inputs from its phosphotyrosine-binding adaptor and Ras to achieve maximal PI(3)K signalling in specific biological situations. |
| Links |
PubMed Online version:10.1038/ncb1493 |
| Keywords |
Animals; Binding Sites/genetics; Brain/drug effects; Brain/growth & development; Brain/metabolism; Cell Survival; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Enzyme Activation/drug effects; Female; Immunoblotting; Insulin/pharmacology; Male; Microscopy, Fluorescence; Mutation/genetics; Phosphatidylinositol 3-Kinases/genetics; Phosphatidylinositol 3-Kinases/metabolism; Phosphorylation/drug effects; Protein Binding; Signal Transduction; Wing/drug effects; Wing/growth & development; Wing/metabolism; ras Proteins/genetics; ras Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
