PMID:16973930

Citation	Gilmour, KM, Bayaa, M, Kenney, L, McNeill, B and Perry, SF (2007) Type IV carbonic anhydrase is present in the gills of spiny dogfish (Squalus acanthias). Am. J. Physiol. Regul. Integr. Comp. Physiol. 292:R556-67
Abstract	Physiological and biochemical studies have provided indirect evidence for a membrane-associated carbonic anhydrase (CA) isoform, similar to mammalian type IV CA, in the gills of dogfish (Squalus acanthias). This CA isoform is linked to the plasma membrane of gill epithelial cells by a glycosylphosphatidylinositol anchor and oriented toward the plasma, such that it can catalyze the dehydration of plasma HCO(3)(-) ions. The present study directly tested the hypothesis that CA IV is present in dogfish gills in a location amenable to catalyzing plasma HCO(3)(-) dehydration. Homology cloning techniques were used to assemble a 1,127 base pair cDNA that coded for a deduced protein of 306 amino acids. Phylogenetic analysis suggested that this protein was a type IV CA. For purposes of comparison, a second cDNA (1,107 base pairs) was cloned from dogfish blood; it encoded a deduced protein of 260 amino acids that was identified as a cytosolic CA through phylogenetic analysis. Using real-time PCR and in situ hybridization, mRNA expression for the dogfish type IV CA was detected in gill tissue and specifically localized to pillar cells and branchial epithelial cells that flanked the pillar cells. Immunohistochemistry using a polyclonal antibody raised against rainbow trout type IV CA revealed a similar pattern of CA IV immunoreactivity and demonstrated a limited degree of colocalization with Na(+)-K(+)-ATPase immunoreactivity. The presence and localization of a type IV CA isoform in the gills of dogfish is consistent with the hypothesis that branchial membrane-bound CA with an extracellular orientation contributes to CO(2) excretion in dogfish by catalyzing the dehydration of plasma HCO(3)(-) ions.
Links	PubMed Online version:10.1152/ajpregu.00477.2006
Keywords	Amino Acid Sequence; Animals; Blotting, Western; Carbonic Anhydrase IV/metabolism; Cloning, Molecular; DNA, Complementary/biosynthesis; DNA, Complementary/genetics; Gills/enzymology; Immunohistochemistry; In Situ Hybridization; Molecular Sequence Data; Phylogeny; RNA, Messenger/biosynthesis; RNA, Messenger/genetics; Reverse Transcriptase Polymerase Chain Reaction; Squalus/metabolism; Tissue Distribution
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