GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16962805

From GONUTS
Jump to: navigation, search
Citation

Lengronne, A, McIntyre, J, Katou, Y, Kanoh, Y, Hopfner, KP, Shirahige, K and Uhlmann, F (2006) Establishment of sister chromatid cohesion at the S. cerevisiae replication fork. Mol. Cell 23:787-99

Abstract

Two identical sister copies of eukaryotic chromosomes are synthesized during S phase. To facilitate their recognition as pairs for segregation in mitosis, sister chromatids are held together from their synthesis onward by the chromosomal cohesin complex. Replication fork progression is thought to be coupled to establishment of sister chromatid cohesion, facilitating identification of replication products, but evidence for this has remained circumstantial. Here we show that three proteins required for sister chromatid cohesion, Eco1, Ctf4, and Ctf18, are found at, and Ctf4 travels along chromosomes with, replication forks. The ring-shaped cohesin complex is loaded onto chromosomes before S phase in an ATP hydrolysis-dependent reaction. Cohesion establishment during DNA replication follows without further cohesin recruitment and without need for cohesin to re-engage an ATP hydrolysis motif that is critical for its initial DNA binding. This provides evidence for cohesion establishment in the context of replication forks and imposes constraints on the mechanism involved.

Links

PubMed Online version:10.1016/j.molcel.2006.08.018

Keywords

Acetyltransferases/analysis; Acetyltransferases/metabolism; Acetyltransferases/physiology; Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/metabolism; Amino Acid Motifs/physiology; Cell Cycle Proteins/analysis; Cell Cycle Proteins/metabolism; Chromatids/metabolism; Chromosomal Proteins, Non-Histone/analysis; Chromosomal Proteins, Non-Histone/metabolism; DNA Replication/physiology; DNA, Fungal/biosynthesis; DNA-Binding Proteins/analysis; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Models, Genetic; Nuclear Proteins/analysis; Nuclear Proteins/metabolism; Nuclear Proteins/physiology; Proliferating Cell Nuclear Antigen/metabolism; S Phase/physiology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/analysis; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.