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PMID:16943275
| Citation |
Bae, YK, Qin, H, Knobel, KM, Hu, J, Rosenbaum, JL and Barr, MM (2006) General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia. Development 133:3859-70 |
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| Abstract |
Ciliary localization of the transient receptor potential polycystin 2 channel (TRPP2/PKD-2) is evolutionarily conserved, but how TRPP2 is targeted to cilia is not known. In this study, we characterize the motility and localization of PKD-2, a TRPP2 homolog, in C. elegans sensory neurons. We demonstrate that GFP-tagged PKD-2 moves bidirectionally in the dendritic compartment. Furthermore, we show a requirement for different molecules in regulating the ciliary localization of PKD-2. PKD-2 is directed to moving dendritic particles by the UNC-101/adaptor protein 1 (AP-1) complex. When expressed in non-native neurons, PKD-2 remains in cell bodies and is not observed in dendrites or cilia, indicating that cell-type specific factors are required for directing PKD-2 to the dendrite. PKD-2 stabilization in cilia and cell bodies requires LOV-1, a functional partner and a TRPP1 homolog. In lov-1 mutants, PKD-2 is greatly reduced in cilia and forms abnormal aggregates in neuronal cell bodies. Intraflagellar transport (IFT) is not essential for PKD-2 dendritic motility or access to the cilium, but may regulate PKD-2 ciliary abundance. We propose that both general and cell-type-specific factors govern TRPP2/PKD-2 subcellular distribution by forming at least two steps involving somatodendritic and ciliary sorting decisions. |
| Links |
PubMed Online version:10.1242/dev.02555 |
| Keywords |
Adaptor Protein Complex 1/metabolism; Animals; Caenorhabditis elegans/metabolism; Caenorhabditis elegans/ultrastructure; Caenorhabditis elegans Proteins/analysis; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Cilia/chemistry; Cilia/metabolism; Cilia/ultrastructure; Dendrites/chemistry; Dendrites/metabolism; Dendrites/ultrastructure; Endoplasmic Reticulum/chemistry; Endoplasmic Reticulum/metabolism; Female; Flagella/chemistry; Flagella/metabolism; Green Fluorescent Proteins/analysis; Male; Neurons, Afferent/chemistry; Neurons, Afferent/metabolism; Neurons, Afferent/ultrastructure; Protein Transport; TRPP Cation Channels/analysis; TRPP Cation Channels/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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