GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:16929305
| Citation |
King, MC, Lusk, CP and Blobel, G (2006) Karyopherin-mediated import of integral inner nuclear membrane proteins. Nature 442:1003-7 |
|---|---|
| Abstract |
Targeting of newly synthesized integral membrane proteins to the appropriate cellular compartment is specified by discrete sequence elements, many of which have been well characterized. An understanding of the signals required to direct integral membrane proteins to the inner nuclear membrane (INM) remains a notable exception. Here we show that integral INM proteins possess basic sequence motifs that resemble 'classical' nuclear localization signals. These sequences can mediate direct binding to karyopherin-alpha and are essential for the passage of integral membrane proteins to the INM. Furthermore, karyopherin-alpha, karyopherin-beta1 and the Ran GTPase cycle are required for INM targeting, underscoring parallels between mechanisms governing the targeting of integral INM proteins and soluble nuclear transport. We also provide evidence that specific nuclear pore complex proteins contribute to this process, suggesting a role for signal-mediated alterations in the nuclear pore complex to allow for passage of INM proteins along the pore membrane. |
| Links |
PubMed Online version:10.1038/nature05075 |
| Keywords |
Amino Acid Motifs; GTP Phosphohydrolases/metabolism; Karyopherins/metabolism; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Nuclear Envelope/metabolism; Nuclear Localization Signals; Nuclear Pore/chemistry; Nuclear Pore/metabolism; Nuclear Pore Complex Proteins/genetics; Nuclear Pore Complex Proteins/metabolism; Protein Binding; Protein Transport; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; ran GTP-Binding Protein/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
