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PMID:16920111
| Citation |
Kong, JS, Kang, JY, Kim, HL, Kwon, OS, Lee, KH and Park, YS (2006) 6-Pyruvoyltetrahydropterin synthase orthologs of either a single or dual domain structure are responsible for tetrahydrobiopterin synthesis in bacteria. FEBS Lett. 580:4900-4 |
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| Abstract |
6-Pyruvoyltetrahydropterin synthase (PTPS) catalyzes the second step of tetrahydrobiopterin (BH4) synthesis. We previously identified PTPS orthologs (bPTPS-Is) in bacteria which do not produce BH4. In this study we disrupted the gene encoding bPTPS-I in Synechococcus sp. PCC 7942, which produces BH4-glucoside. The mutant was normal in BH4-glucoside production, demonstrating that bPTPS-I does not participate in BH4 synthesis in vivo and bringing us a new PTPS ortholog (bPTPS-II) of a bimodular polypeptide. The recombinant Synechococcus bPTPS-II was assayed in vitro to show PTPS activity higher than human enzyme. Further computational analysis revealed the presence of mono and bimodular bPTPS-II orthologs mostly in green sulfur bacteria and cyanobacteria, respectively, which are well known for BH4-glycoside production. In summary we found new bacterial PTPS orthologs, having either a single or dual domain structure and being responsible for BH4 synthesis in vivo, thereby disclosing all the bacterial PTPS homologs. |
| Links |
PubMed Online version:10.1016/j.febslet.2006.08.006 |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/classification; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biopterin/analogs & derivatives; Biopterin/biosynthesis; Humans; Isoenzymes/chemistry; Isoenzymes/classification; Isoenzymes/genetics; Isoenzymes/metabolism; Molecular Sequence Data; Mutagenesis; Phosphorus-Oxygen Lyases/chemistry; Phosphorus-Oxygen Lyases/classification; Phosphorus-Oxygen Lyases/genetics; Phosphorus-Oxygen Lyases/metabolism; Phylogeny; Protein Conformation; Recombinant Proteins/chemistry; Recombinant Proteins/classification; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Alignment; Synechococcus/genetics; Synechococcus/metabolism |
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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