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PMID:16915353
Citation |
Kim, SK, Mason, JT, Knaff, DB, Bauer, CE and Setterdahl, AT (2006) Redox properties of the Rhodobacter sphaeroides transcriptional regulatory proteins PpsR and AppA. Photosyn. Res. 89:89-98 |
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Abstract |
Redox properties of the photosynthetic gene repressor PpsR and the blue-light photoreceptor/antirepressor AppA from Rhodobacter sphaeroides have been characterized. Redox titrations of PpsR reveal the presence of a two-electron couple, with an E (m) value of -320 mV at pH 7.0, which is likely to arise from the reversible conversion of two cysteine thiols to a disulfide. This E (m) value is very much more negative than the E (m) = -180 mV value measured previously at pH 7.0 for the disulfide/dithiol couple in CrtJ, the homolog for PpsR in the closely related bacterium Rhodobacter capsulatus. AppA, a flavin-containing blue-light receptor that is also involved in the regulation of gene expression in R. sphaeroides, contains multiple cysteines in its C-terminal region, two of which function as a redox-active dithiol/disulfide couple with an E (m) value of -325 mV at pH 7.0 in the dark. Titrations of this dithiol/disulfide couple in illuminated samples of AppA indicate that the E (m) value of this disulfide/dithiol couple is -315 mV at pH 7.0, identical to the value obtained for AppA in the dark within the combined experimental uncertainties of the two measurements. The E (m) values of AppA and PpsR demonstrate that these proteins are thermodynamically capable of electron transfer for their activity as an anti-repressor/repressor in R. sphaeroides. |
Links |
PubMed PMC2774731 Online version:10.1007/s11120-006-9086-4 |
Keywords |
Bacterial Proteins/metabolism; DNA-Binding Proteins/metabolism; Flavoproteins/metabolism; Gene Expression Regulation, Bacterial; Oxidation-Reduction; Repressor Proteins/metabolism; Rhodobacter sphaeroides/genetics; Rhodobacter sphaeroides/metabolism; Transcription, Genetic |
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Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
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See also
References
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