PMID:1690859

Citation	Schöler, HR, Ruppert, S, Suzuki, N, Chowdhury, K and Gruss, P (1990) New type of POU domain in germ line-specific protein Oct-4. Nature 344:435-9
Abstract	Members of a family of murine octamer-binding proteins interact specifically with the octamer motif, a transcription regulatory element found in the promoter and enhancer regions of many genes. Oct-4 is a maternally expressed protein that is also present in the pre-implantation mouse embryo. Although many regulatory proteins are expressed in post-implantation embryos, transcription factors regulating pre-implantation processes have remained elusive. The Oct-4 gene is therefore a prime candidate for an early developmental control gene. Here we report the complementary DNA cloning of the mouse Oct-4 gene, and the characterization of the encoded protein(s) by sequential in vitro transcription, translation, DNA-binding and protease-clipping analysis. Deletion analysis shows that the DNA-binding activity is mediated by a POU domain encoded in an open reading frame corresponding to a 324-amino-acid protein. Sequence comparison with known POU domains reveals that Oct-4 is a novel member of the POU-family.
Links	PubMed Online version:10.1038/344435a0
Keywords	Animals; Binding Sites; Blastocyst/analysis; Cloning, Molecular; DNA/genetics; DNA/metabolism; DNA-Binding Proteins/analysis; DNA-Binding Proteins/genetics; Germ Cells/analysis; Mice; Octamer Transcription Factor-3; Oocytes/analysis; Peptide Hydrolases; Protein Biosynthesis; RNA/analysis; Sequence Homology, Nucleic Acid; Tissue Distribution; Transcription Factors; Transcription, Genetic
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