PMID:16891302

Citation	Suzuki, H, Takahashi, S, Watanabe, R, Fukushima, Y, Fujita, N, Noguchi, A, Yokoyama, R, Nishitani, K, Nishino, T and Nakayama, T (2006) An isoflavone conjugate-hydrolyzing beta-glucosidase from the roots of soybean (Glycine max) seedlings: purification, gene cloning, phylogenetics, and cellular localization. J. Biol. Chem. 281:30251-9
Abstract	Soybeans (Glycine max (L.) Merr.) and certain other legumes excrete isoflavones from their roots, which participate in plantmicrobe interactions such as symbiosis and as a defense against infections by pathogens. In G. max, the release of free isoflavones from their conjugates, the latent forms, is mediated by an isoflavone conjugate-hydrolyzing beta-glucosidase. Here we report on the purification and cDNA cloning of this important beta-glucosidase from the roots of G. max seedlings as well as related phylogenetic and cellular localization studies. The purified enzyme, isoflavone conjugate-hydrolyzing beta-glucosidase from roots of G. max seedling (GmICHG), is a homodimeric glycoprotein with a subunit molecular mass of 58 kDa and is capable of directly hydrolyzing genistein 7-O-(6 -O-malonyl-beta-d-glucoside) to produce free genistein (k(cat), 98 s(-1); K(m), 25 microM at 30 degrees C, pH 7.0). GmICHG cDNA was isolated based on the amino acid sequence of the purified enzyme. GmICHG cDNA was abundantly expressed in the roots of G. max seedlings but only negligibly in the hypocotyl and cotyledon. An immunocytochemical analysis using anti-GmICHG antibodies, along with green fluorescent protein imaging analyses of Arabidopsis cultured cells transformed by the GmICHG:GFP fusion gene, revealed that the enzyme is exclusively localized in the cell wall and intercellular space of seedling roots, particularly in the cell wall of root hairs. A phylogenetic analysis revealed that GmICHG is a member of glycoside hydrolase family 1 and can be co-clustered with many other leguminous beta-glucosidases, the majority of which may also be involved in flavonoid-mediated interactions of legumes with microbes.
Links	PubMed Online version:10.1074/jbc.M605726200
Keywords	Cells, Cultured; Cloning, Molecular; Cotyledon/cytology; Cotyledon/enzymology; Cotyledon/genetics; Hydrolysis; Hypocotyl/cytology; Hypocotyl/enzymology; Hypocotyl/genetics; Isoflavones/metabolism; Molecular Sequence Data; Phylogeny; Plant Roots/cytology; Plant Roots/enzymology; Plant Roots/genetics; Seedling/cytology; Seedling/enzymology; Seedling/genetics; Seedling/metabolism; Soybeans/cytology; Soybeans/enzymology; Soybeans/genetics; Soybeans/metabolism; beta-Glucosidase/genetics; beta-Glucosidase/isolation & purification; beta-Glucosidase/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
SOYBN:Q08IT7	part_of	GO:0005618: cell wall	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
SOYBN:Q08IT7		GO:0005618: cell wall	ECO:0000314:		C		Figure 5	complete CACAO 8361

References

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