PMID:16890369

Citation	Luvisetto, S, Marinelli, S, Panasiti, MS, D'Amato, FR, Fletcher, CF, Pavone, F and Pietrobon, D (2006) Pain sensitivity in mice lacking the Ca(v)2.1alpha1 subunit of P/Q-type Ca2+ channels. Neuroscience 142:823-32
Abstract	The role of voltage-gated Ca(2+) (Ca(V)) channels in pain mechanisms has been the object of intense investigation using pharmacological approaches and, more recently, using mutant mouse models lacking the Ca(V)alpha(l) pore-forming subunit of N-, R- and T-type channels. The role of P/Q-type channels in nociception and pain transmission has been investigated by pharmacological approaches but remains to be fully elucidated. To address this issue, we have analyzed pain-related behavioral responses of null mutant mice for the Ca(V)2.1alpha(1) subunit of P/Q-type channels. Homozygous null mutant Ca(V)2.1alpha(1)-/- mice developed dystonia at 10-12 days after birth and did not survive past weaning. Tested at ages where motor deficit was either absent or very mild, Ca(V)2.1alpha(1)-/- mice showed reduced tail withdrawal latencies in the tail-flick test and reduced abdominal writhes in the acetic acid writhing test. Adult heterozygous Ca(V)2.1alpha(1)+/- mice did not show motor deficits in the rotarod and activity cage tests and did not show alterations in pain responses in the tail-flick test and the acetic acid writhing test. Strikingly, they showed a reduced licking response during the second phase of formalin-induced inflammatory pain and a reduced mechanical allodynia in the chronic constriction injury model of neuropathic pain. Our findings show that P/Q-type channels play an antinociceptive role in sensitivity to non-injurious noxious thermal stimuli and a pronociceptive role in inflammatory and neuropathic pain states, pointing to an important role of Ca(V)2.1 channels in central sensitization.
Links	PubMed Online version:10.1016/j.neuroscience.2006.06.049
Keywords	Analysis of Variance; Animals; Animals, Newborn; Behavior, Animal; Calcium Channels, N-Type/deficiency; Female; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Motor Activity/genetics; Pain/genetics; Pain Measurement/methods; Pain Threshold/physiology; Protein Subunits/genetics; Psychomotor Performance/physiology; Reaction Time/genetics; Time Factors
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