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PMID:16856985
| Citation |
Shimotohno, A, Ohno, R, Bisova, K, Sakaguchi, N, Huang, J, Koncz, C, Uchimiya, H and Umeda, M (2006) Diverse phosphoregulatory mechanisms controlling cyclin-dependent kinase-activating kinases in Arabidopsis. Plant J. 47:701-10 |
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| Abstract |
For the full activation of cyclin-dependent kinases (CDKs), not only cyclin binding but also phosphorylation of a threonine (Thr) residue within the T-loop is required. This phosphorylation is catalyzed by CDK-activating kinases (CAKs). In Arabidopsis three D-type CDK genes (CDKD;1-CDKD;3) encode vertebrate-type CAK orthologues, of which CDKD;2 exhibits high phosphorylation activity towards the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. Here, we show that CDKD;2 forms a stable complex with cyclin H and is downregulated by the phosphorylation of the ATP-binding site by WEE1 kinase. A knockout mutant of CDKD;3, which has a higher CDK kinase activity, displayed no defect in plant development. Instead, another type of CAK - CDKF;1 - exhibited significant activity towards CDKA;1 in Arabidopsis root protoplasts, and the activity was dependent on the T-loop phosphorylation of CDKF;1. We propose that two distinct types of CAK, namely CDKF;1 and CDKD;2, play a major role in CDK and CTD phosphorylation, respectively, in Arabidopsis. |
| Links |
PubMed Online version:10.1111/j.1365-313X.2006.02820.x |
| Keywords |
Amino Acid Sequence; Arabidopsis/anatomy & histology; Arabidopsis/enzymology; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Cyclin H; Cyclin-Dependent Kinases/genetics; Cyclin-Dependent Kinases/metabolism; Cyclin-Dependent Kinases/physiology; Cyclins/metabolism; Down-Regulation; Models, Biological; Molecular Sequence Data; Phosphorylation; Plant Roots/cytology; Plant Roots/enzymology; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Protein-Serine-Threonine Kinases/physiology; Protoplasts/cytology; Protoplasts/enzymology; Sequence Alignment; Tyrosine/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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