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PMID:16825665
| Citation |
Myers, GS, Rasko, DA, Cheung, JK, Ravel, J, Seshadri, R, DeBoy, RT, Ren, Q, Varga, J, Awad, MM, Brinkac, LM, Daugherty, SC, Haft, DH, Dodson, RJ, Madupu, R, Nelson, WC, Rosovitz, MJ, Sullivan, SA, Khouri, H, Dimitrov, GI, Watkins, KL, Mulligan, S, Benton, J, Radune, D, Fisher, DJ, Atkins, HS, Hiscox, T, Jost, BH, Billington, SJ, Songer, JG, McClane, BA, Titball, RW, Rood, JI, Melville, SB and Paulsen, IT (2006) Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens. Genome Res. 16:1031-40 |
|---|---|
| Abstract |
Clostridium perfringens is a Gram-positive, anaerobic spore-forming bacterium commonly found in soil, sediments, and the human gastrointestinal tract. C. perfringens is responsible for a wide spectrum of disease, including food poisoning, gas gangrene (clostridial myonecrosis), enteritis necroticans, and non-foodborne gastrointestinal infections. The complete genome sequences of Clostridium perfringens strain ATCC 13124, a gas gangrene isolate and the species type strain, and the enterotoxin-producing food poisoning strain SM101, were determined and compared with the published C. perfringens strain 13 genome. Comparison of the three genomes revealed considerable genomic diversity with >300 unique "genomic islands" identified, with the majority of these islands unusually clustered on one replichore. PCR-based analysis indicated that the large genomic islands are widely variable across a large collection of C. perfringens strains. These islands encode genes that correlate to differences in virulence and phenotypic characteristics of these strains. Significant differences between the strains include numerous novel mobile elements and genes encoding metabolic capabilities, strain-specific extracellular polysaccharide capsule, sporulation factors, toxins, and other secreted enzymes, providing substantial insight into this medically important bacterial pathogen. |
| Links |
PubMed PMC1524862 Online version:10.1101/gr.5238106 |
| Keywords |
Bacterial Toxins; Base Sequence; Clostridium perfringens/genetics; DNA, Bacterial; Genome, Bacterial; Molecular Sequence Data; Polymerase Chain Reaction |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016787: hydrolase activity |
ECO:0000314: |
F |
Matched 64.3% with Dual specificity protein (Crystal Structure of human MKP-2). Figure 3 in the PubMed article shows the shift in gel mobility assay VirR binding to novel putative boxes from C. perfringens ATCC 13124 and SM101. Diffirent amounts of VirR protein were used in model A whereas no protein or a set amount of protein was used in model B. The control gene was identified as the pfoA gene from strain 13. |
complete | ||||
Notes
See also
References
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