PMID:16790495

Citation	Mercer, KB, Miller, RK, Tinley, TL, Sheth, S, Qadota, H and Benian, GM (2006) Caenorhabditis elegans UNC-96 is a new component of M-lines that interacts with UNC-98 and paramyosin and is required in adult muscle for assembly and/or maintenance of thick filaments. Mol. Biol. Cell 17:3832-47
Abstract	To gain further insight into the molecular architecture, assembly, and maintenance of the sarcomere, we have carried out a molecular analysis of the UNC-96 protein in the muscle of Caenorhabditis elegans. By polarized light microscopy of body wall muscle, unc-96 mutants display reduced myofibrillar organization and characteristic birefringent "needles." By immunofluorescent staining of known myofibril components, unc-96 mutants show major defects in the organization of M-lines and in the localization of a major thick filament component, paramyosin. In unc-96 mutants, the birefringent needles, which contain both UNC-98 and paramyosin, can be suppressed by starvation or by exposure to reduced temperature. UNC-96 is a novel approximately 47-kDa polypeptide that has no recognizable domains. Antibodies generated to UNC-96 localize the protein to the M-line, a region of the sarcomere in which thick filaments are cross-linked. By genetic and biochemical criteria, UNC-96 interacts with UNC-98, a previously described component of M-lines, and paramyosin. Additionally, UNC-96 copurifies with native thick filaments. A model is presented in which UNC-96 is required in adult muscle to promote thick filament assembly and/or maintenance.
Links	PubMed PMC1593161 Online version:10.1091/mbc.E06-02-0144
Keywords	Actin Cytoskeleton/metabolism; Actin Cytoskeleton/ultrastructure; Amino Acid Sequence; Animals; Antibodies/immunology; Caenorhabditis elegans/cytology; Caenorhabditis elegans/embryology; Caenorhabditis elegans/metabolism; Caenorhabditis elegans/ultrastructure; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Genes, Helminth/genetics; Molecular Sequence Data; Movement/physiology; Muscle Proteins/chemistry; Muscle Proteins/metabolism; Muscles/cytology; Muscles/metabolism; Muscles/ultrastructure; Mutation/genetics; Myofibrils/ultrastructure; Peptides/chemistry; Peptides/metabolism; Phenotype; Protein Binding; Protein Isoforms/chemistry; Protein Isoforms/metabolism; Protein Structure, Tertiary; Protein Transport; Temperature; Tropomyosin/metabolism
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