PMID:16781730

Citation	Surtees, JA and Alani, E (2006) Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination. J. Mol. Biol. 360:523-36
Abstract	Genetic studies in Saccharomyces cerevisiae predict that the mismatch repair (MMR) factor MSH2-MSH3 binds and stabilizes branched recombination intermediates that form during single strand annealing and gene conversion. To test this model, we constructed a series of DNA substrates that are predicted to form during these recombination events. We show in an electrophoretic mobility shift assay that S. cerevisiae MSH2-MSH3 specifically binds branched DNA substrates containing 3' single-stranded DNA and that ATP stimulates its release from these substrates. Chemical footprinting analyses indicate that MSH2-MSH3 specifically binds at the double-strand/single-strand junction of branched substrates, alters its conformation and opens up the junction. Therefore, MSH2-MSH3 binding to its substrates creates a unique nucleoprotein structure that may signal downstream steps in repair that include interactions with MMR and nucleotide excision repair factors.
Links	PubMed Online version:10.1016/j.jmb.2006.05.032
Keywords	Adenosine Triphosphate/metabolism; Base Sequence; DNA Repair/genetics; DNA, Fungal/chemistry; DNA, Fungal/genetics; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/metabolism; Fungal Proteins/metabolism; Molecular Sequence Data; MutS Homolog 2 Protein/metabolism; Nucleic Acid Conformation; Protein Binding; Recombination, Genetic; Saccharomyces cerevisiae Proteins/metabolism; Substrate Specificity
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