PMID:16781467

Citation	Suzuki, M, Hirai, T, Arai, H, Ishii, M and Igarashi, Y (2006) Purification, characterization, and gene cloning of thermophilic cytochrome cd1 nitrite reductase from Hydrogenobacter thermophilus TK-6. J. Biosci. Bioeng. 101:391-7
Abstract	A thermophilic, chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6 can grow autotrophically under anaerobic conditions by denitrification. One of the denitrification enzymes, cytochrome cd(1) nitrite reductase, was isolated and its gene was cloned from strain TK-6. The subunit molecular mass of the purified enzyme was 61.5 kDa and the isoelectric point was determined to be 9.3. The optimum temperature and pH for the enzymatic reaction were 70-75 degrees C and 6.5-7.0, respectively. The structural gene for the enzyme, nirS, is probably transcribed as a hexacistronic operon with the following genes encoding a putative diheme cytochrome c and the proteins required for biosynthesis of heme d(1). The NirS sequence was phylogenetically distinct from those of proteobacteria. The consensus -35 and -10 sequences were found in the putative nirS promoter region, but the consensus sequences for the DNR/NnrR-type or the NorR/FhpR-type nitric oxide sensing regulators were not found in this region.
Links	PubMed Online version:10.1263/jbb.101.391
Keywords	Amino Acid Sequence; Bacteria/enzymology; Cloning, Molecular; Cytochromes/chemistry; Cytochromes/genetics; Cytochromes/isolation & purification; Cytochromes/metabolism; Enzyme Activation; Enzyme Stability; Molecular Sequence Data; Molecular Weight; Nitrite Reductases/chemistry; Nitrite Reductases/genetics; Nitrite Reductases/isolation & purification; Nitrite Reductases/metabolism; Protein Engineering/methods; Sequence Homology, Amino Acid; Temperature
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HYDTH:Q3LGA9	enables	GO:0050421: nitrite reductase (NO-forming) activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HYDTH:Q3LGA9		GO:0050421: nitrite reductase (NO-forming) activity	ECO:0000314:		F		Table 1.	complete CACAO 8986

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