GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

It is now the 1st OPEN Period for CACAO Fall 2019! It will end on Sunday September 22, 2019 at 11:59 pm CDT
This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.
Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

Thomason, PA, Brazill, DT and Cox, EC (2006) A series of Dictyostelium expression vectors for recombination cloning. Plasmid 56:145-52


We describe a series of Dictyostelium expression vectors for recombination cloning using the Gateway technology. DNA fragments generated by high fidelity polymerase chain reaction are cloned by topoisomerase-mediated ligation, then recombined into any of several Dictyostelium expression vectors using phage lambda LR recombinase. No restriction enzymes are used in this procedure. Coding regions can be expressed from their own promoters, or from a strong actin 15 promoter as a native protein, or with an amino or carboxyl-terminal GFP fusion. Gene promoters of interest can be analyzed by controlled expression of GFP and beta-galactosidase. These vectors allow for rapid and simple characterization of novel DNA, and are ideal for high-throughput studies.


PubMed Online version:10.1016/j.plasmid.2006.04.001


Animals; Bacteriophage lambda; Base Sequence; Cloning, Molecular/methods; DNA Primers; DNA Topoisomerases; Dictyostelium/genetics; Gene Expression; Genetic Vectors/genetics; Green Fluorescent Proteins; Molecular Sequence Data; Polymerase Chain Reaction/methods; Promoter Regions, Genetic/genetics; beta-Galactosidase



Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

See also


See Help:References for how to manage references in GONUTS.