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PMID:1674449
| Citation |
Hedley, ML and Maniatis, T (1991) Sex-specific splicing and polyadenylation of dsx pre-mRNA requires a sequence that binds specifically to tra-2 protein in vitro. Cell 65:579-86 |
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| Abstract |
Somatic sex determination in Drosophila involves a hierarchy of regulated alternative pre-mRNA processing. Female-specific splicing and/or polyadenylation of doublesex (dsx) pre-mRNA, the final gene in this pathway, requires transformer (tra) and transformer-2 (tra-2) proteins. The mechanisms by which these proteins regulate RNA processing has not been characterized. In this paper we show that tra-2 produced in Escherichia coli binds specifically to a site within the female-specific exon of dsx pre-mRNA. This site, which contains six copies of a 13 nucleotide repeat, is required not only for female-specific splicing, but also for female-specific polyadenylation. These observations suggest that tra-2 is a positive regulator of dsx pre-mRNA processing. |
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| Keywords |
Animals; Base Sequence; Binding Sites; Cell Line; Cloning, Molecular; Drosophila/genetics; Drosophila/physiology; Drosophila Proteins; Exons; Female; Male; Molecular Sequence Data; Plasmids; Poly A/genetics; RNA Precursors/genetics; RNA Precursors/metabolism; RNA Probes; RNA Splicing; RNA, Messenger/genetics; Repetitive Sequences, Nucleic Acid; Ribonucleoproteins/metabolism; Sex Determination Analysis; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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