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PMID:1674354
| Citation |
Mill, JF, Mearow, KM, Purohit, HJ, Haleem-Smith, H, King, R and Freese, E (1991) Cloning and functional characterization of the rat glutamine synthetase gene. Brain Res. Mol. Brain Res. 9:197-207 |
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| Abstract |
Glutamine synthetase catalyzes the formation of glutamine from glutamate and ammonia. It plays a central role in both amino acid neurotransmitter metabolism and ammonia detoxification in the central nervous system. Glutamine synthetase expression is regulated in developmental, hormonal, and in tissue- and cell-specific manners. We have cloned a full-length cDNA coding for rat glutamine synthetase, and have found an AT-rich area of conservation in the 3' untranslated regions between rat, mouse, and chicken, which may play a part in the regulation of the stability of the glutamine synthetase message. We have also cloned and mapped the gene coding for rat glutamine synthetase, and identified, by sequence analysis, areas potentially important for the regulation of glutamine synthetase transcription. Transient transfection of a variety of cell lines with deletion constructs of the glutamine synthetase promoter driving a chloramphenicol acetyltransferase reporter gene functionally demonstrates regions of the promoter containing elements important for transcriptional regulation. |
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| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Chloramphenicol O-Acetyltransferase/genetics; Cloning, Molecular; Gene Expression Regulation, Enzymologic/physiology; Glutamate-Ammonia Ligase/genetics; Luciferases/analysis; Molecular Sequence Data; Promoter Regions, Genetic/genetics; Rats; Transfection/genetics; Tumor Cells, Cultured |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0042133: neurotransmitter metabolic process |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019676: ammonia assimilation cycle |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004356: glutamate-ammonia ligase activity |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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