PMID:16730786

Citation	Kida, K, Baba, E, Torii, R, Kawate, N, Hatoya, S, Wijewardana, V, Sugiura, K, Sawada, T, Tamada, H and Inaba, T (2006) Lactoferrin expression in the canine uterus during the estrous cycle and with pyometra. Theriogenology 66:1325-33
Abstract	The expression of lactoferrin, a non-specific antimicrobial defence, in the canine uterus during the normal estrous cycle and in bitches with pyometra was examined. Using polymerase chain reaction analysis, lactoferrin gene transcripts were detected in the endometrium at all stages of the estrous cycle, with the highest levels in estrus. In normal bitches, endometrial lactoferrin mRNA increased from proestrus to estrus (P<0.05). Thereafter, it dramatically decreased from estrus to Day 10 of diestrus (P<0.05), and stayed low at Day 35 of diestrus and anestrus; this was consistent with blood estrogen concentrations. Levels of lactoferrin mRNA were higher in bitches with pyometra than in normal diestrus (P<0.05). With immunohistochemistry, distinct staining of lactoferrin was detected in the luminal and glandular epithelial cells of the endometrium at proestrus and estrus, but little staining was detected at Day 10 of diestrus. At Day 35 of diestrus and anestrus, a partial and weak reaction was present in the same region. In bitches with pyometra, the glandular epithelial cells and many cells in the uterine stroma were strongly stained. Staining cells in the stroma were morphologically similar to neutrophils. No lactoferrin staining was seen in the uterine stromal cells or myometrium in any section. These results suggest that, in the canine uterus, lactoferrin expression is related to the blood concentration of estrogen, and that the dramatic reduction in lactoferrin observed at the early stage of diestrus may impair antimicrobial defense. Also, enhanced expression of lactoferrin mRNA in the endometrium with pyometra may be associated with neutrophil invasion into the uterus to combat the infection.
Links	PubMed Online version:10.1016/j.theriogenology.2006.04.028
Keywords	Amino Acid Sequence; Animals; Base Sequence; Dog Diseases/metabolism; Dogs; Estrogens/blood; Estrus/metabolism; Estrus/physiology; Female; Gene Expression; Immunohistochemistry/veterinary; Lactoferrin/genetics; Lactoferrin/metabolism; Molecular Sequence Data; Polymerase Chain Reaction/methods; Polymerase Chain Reaction/veterinary; RNA, Messenger/metabolism; Sequence Alignment/veterinary; Uterine Diseases/metabolism; Uterine Diseases/veterinary; Uterus/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CANFA:Q2LC20		GO:0033571: lactoferrin transport	ECO:0000314:		P		Figure 5. Lactoferrin will change as an immune response to a pyometra infection.	complete CACAO 3526

References

See Help:References for how to manage references in GONUTS.