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PMID:16702557

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Citation

Dong, CH, Agarwal, M, Zhang, Y, Xie, Q and Zhu, JK (2006) The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1. Proc. Natl. Acad. Sci. U.S.A. 103:8281-6

Abstract

Plant responses to cold stress are mediated by a transcriptional cascade, in which the transcription factor ICE1 and possibly related proteins activate the expression of C-repeat (CRT)-binding factors (CBFs), leading to the transcription of downstream effector genes. The variant RING finger protein high expression of osmotically responsive gene (HOS)1 was identified genetically as a negative regulator of cold responses. We present evidence here that HOS1 is an E3 ligase required for the ubiquitination of ICE1. HOS1 physically interacts with ICE1 and mediates the ubiquitination of ICE1 both in vitro and in vivo. We found that cold induces the degradation of ICE1 in plants, and this degradation requires HOS1. Consistent with enhanced cold-responsive gene expression in loss-of-function hos1 mutant plants, overexpression of HOS1 represses the expression of CBFs and their downstream genes and confers increased sensitivity to freezing stress. Our results indicate that cold stress responses in Arabidopsis are attenuated by a ubiquitination/proteasome pathway in which HOS1 mediates the degradation of the ICE1 protein.

Links

PubMed PMC1472463 Online version:10.1073/pnas.0602874103

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/physiology; Cold Temperature; Gene Expression Regulation, Plant; Intracellular Signaling Peptides and Proteins/physiology; Nuclear Proteins/physiology; Plant Physiological Phenomena; Plant Proteins/metabolism; Plants, Genetically Modified; Protein Structure, Tertiary; Temperature; Transcription Factors/metabolism; Transcription Factors/physiology; Two-Hybrid System Techniques; Ubiquitin/chemistry

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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